Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions.

ABSTRACT: Here we present a protocol to study intracellular protein-protein interactions that is based on the widely used biotin-avidin pull-down system. The modification presented includes the combination of this technique with cell-penetrating sequences. We propose to design cell-penetrating baits that can be incubated with living cells instead of cell lysates and therefore the interactions found will reflect those that occur within the intracellular context. Connexin43 (Cx43), a protein that forms gap junction channels and hemichannels is down-regulated in high-grade gliomas. The Cx43 region comprising amino acids 266-283 is responsible for the inhibition of the oncogenic activity of c-Src in glioma cells. Here we use TAT as the cell-penetrating sequence, biotin as the pull-down tag and the regio

SUBMITTER: Jaraiz-Rodriguez M 

PROVIDER: S-EPMC5755618 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature