A permanent window for the murine lung enables high-resolution imaging of cancer metastasis.

ABSTRACT: Stable, high-resolution intravital imaging of the lung has become possible through the utilization of vacuum-stabilized imaging windows. However, this technique is extremely invasive and limited to only hours in duration. Here we describe a minimally invasive, permanently implantable window for high-resolution intravital imaging of the murine lung that allows the mouse to survive surgery, recover from anesthesia, and breathe independently. Compared to vacuum-stabilized windows, this window produces the same high-quality images without vacuum-induced artifacts; it is also less invasive, which allows imaging of the same lung tissue over a period of weeks. We further adapt the technique of microcartography for reliable relocalization of the same cells longitudinally. Using commonly employed e

SUBMITTER: Entenberg D 

PROVIDER: S-EPMC5755704 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature