Development of a biosensor protein bullet as a fluorescent method for fast detection of Escherichia coli in drinking water.

ABSTRACT: Drinking water can be exposed to different biological contaminants from the source, through the pipelines, until reaching the final consumer or industry. Some of these are pathogenic bacteria and viruses which may cause important gastrointestinal or systemic diseases. The microbiological quality of drinking water relies mainly in monitoring three indicator bacteria of faecal origin, Escherichia coli, Enterococcus faecalis and Clostridium perfringens, which serve as early sentinels of potential health hazards for the population. Here we describe the analysis of three chimeric fluorescent protein bullets as biosensor candidates for fast detection of E. coli in drinking water. Two of the chimeric proteins (based on GFP-hadrurin and GFP-pb5 chimera proteins) failed with respect to specificity

SUBMITTER: Gutierrez-Del-Rio I 

PROVIDER: S-EPMC5755745 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature