Transcriptome sequencing and de novo assembly in arecanut, Areca catechu L elucidates the secondary metabolite pathway genes.

ABSTRACT: Areca catechu L. belongs to the Arecaceae family which comprises many economically important palms. The palm is a source of alkaloids and carotenoids. The lack of ample genetic information in public databases has been a constraint for the genetic improvement of arecanut. To gain molecular insight into the palm, high throughput RNA sequencing and de novo assembly of arecanut leaf transcriptome was undertaken in the present study. A total 56,321,907 paired end reads of 101 bp length consisting of 11.343 Gb nucleotides were generated. De novo assembly resulted in 48,783 good quality transcripts, of which 67% of transcripts could be annotated against NCBI non - redundant database. The Gene Ontology (GO) analysis with UniProt database identified 9222 biological process, 11268 molecular f

SUBMITTER: Manimekalai R 

PROVIDER: S-EPMC5755930 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature