Proteomic analysis of low-molecular-weight glutenin subunits and relationship with their genes in a common wheat variety.

ABSTRACT: Although many studies on low-molecular-weight glutenin subunit (LMW-GS) function have been reported, a comprehensive comparison between specific genes and their protein product is still lacking. This study aimed to link the 43 genes isolated from the Korean wheat variety "Jokyoung" in the authors' previous study to their protein products. Proteins were separated using two-dimensional gel electrophoresis (2-DGE) and identified by tandem mass spectrometry (MS/MS) at the gene haplotype level. Using MS/MS analysis of 17 protein spots, two spots were identified in the Glu-A3 locus and the corresponding haplotype was GluA3-13(Glu-A3c). Six spots were identified in the Glu-B3 locus and the corresponding haplotypes were GluB3-33

SUBMITTER: Beom HR 

PROVIDER: S-EPMC5756151 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature