Genotyping Echinococcus granulosus from Canine Isolates in Ilam Province, West of Iran.

ABSTRACT:

Background

Cystic Echinococcosis (CE) is one of the most common parasitic zoonosis caused by Echinococcus granulosus worldwide. This study investigated genotype diversity of Echinococcus granulosus isolated from stray dogs and golden jackals in Ilam province, West of Iran.

Methods

Adult worms were collected from the small intestine of the stray dogs and golden jackals from Ilam Province roads during 2012-2014. DNA was extracted from the adult worms and the partial mitochondrial NADH dehydrogenase subunit1 (nad1) was amplified by PCR then the products were digested by using HpaII, Rsa1, and Alu1 restriction enzymes. In order to confirm RFLP results, a number of PCR products were bi-directionally sequenced.

Results

Totally, 20 stray do

SUBMITTER: Dalimi A 

PROVIDER: S-EPMC5756312 | biostudies-literature | 2017 Oct-Dec

REPOSITORIES: biostudies-literature