RNAseq analysis of α-proteobacterium Gluconobacter oxydans 621H.

ABSTRACT:

Background

The acetic acid bacterium Gluconobacter oxydans 621H is characterized by its exceptional ability to incompletely oxidize a great variety of carbohydrates in the periplasm. The metabolism of this α-proteobacterium has been characterized to some extent, yet little is known about its transcriptomes and related data. In this study, we applied two different RNAseq approaches. Primary transcriptomes enriched for 5'-ends of transcripts were sequenced to detect transcription start sites, which allow subsequent analysis of promoter motifs, ribosome binding sites, and 5´-UTRs. Whole transcriptomes were sequenced to identify expressed genes and operon structures.

Results

Sequencing of primary transcriptomes of G. oxydans revealed 2449 TSSs, which were classified according to

SUBMITTER: Kranz A 

PROVIDER: S-EPMC5756330 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature