Bacterial ribonuclease binase exerts an intra-cellular anti-viral mode of action targeting viral RNAs in influenza a virus-infected MDCK-II cells.

ABSTRACT: BACKGROUND:Influenza is a severe contagious disease especially in children, elderly and immunocompromised patients. Beside vaccination, the discovery of new anti-viral agents represents an important strategy to encounter seasonal and pandemic influenza A virus (IAV) strains. The bacterial extra-cellular ribonuclease binase is a well-studied RNase from Bacillus pumilus. Treatment with binase was shown to improve survival of laboratory animals infected with different RNA viruses. Although binase reduced IAV titer in vitro and in vivo, the mode of action (MOA) of binase against IAV at the molecular level has yet not been studied in depth and remains elusive. METHODS:To analyze whether binase impairs virus replication by direct interaction with the viral particle we applied a hemagglutination

SUBMITTER: Shah Mahmud R 

PROVIDER: S-EPMC5756404 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature