DiskImageR: quantification of resistance and tolerance to antimicrobial drugs using disk diffusion assays.

ABSTRACT: Microbial pathogens represent an increasing threat to human health. Although many infections can be successfully treated and cleared, drug resistance is a widespread problem. The existence of subpopulations of 'tolerant' cells (where a fraction of the population is able to grow above the population resistance level) may increase the rate of treatment failure; yet, existing methods to measure subpopulation effects are cumbersome. Here we describe diskImageR, a computational pipeline that analyses photographs of disk diffusion assays to determine the degree of drug susceptibility [the radius of inhibition, (RAD)], and two aspects of subpopulation growth [the fraction of growth (FoG) within the zone of inhibition, (ZOI), and the rate of change in growth from no drug to inhibitory drug concent

SUBMITTER: Gerstein AC 

PROVIDER: S-EPMC5756480 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature