Identification and characterization of haemofungin, a novel antifungal compound that inhibits the final step of haem biosynthesis.

ABSTRACT:

Objectives

During recent decades, the number of invasive fungal infections among immunosuppressed patients has increased significantly, whereas the number of effective systemic antifungal drugs remains low and unsatisfactory. The aim of this study was to characterize a novel antifungal compound, CW-8/haemofungin, which we previously identified in a screen for compounds affecting fungal cell wall integrity.

Methods

The in vitro characteristics of haemofungin were investigated by MIC evaluation against a panel of pathogenic and non-pathogenic fungi, bacteria and mammalian cells in culture. Haemofungin mode-of-action studies were performed by screening an Aspergillus nidulans overexpression genomic library for resistance-conferring plasmids and biochemical validation of the tar

SUBMITTER: Ben Yaakov D 

PROVIDER: S-EPMC5757792 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature