Revisiting the specificity of the MHC class II transactivator CIITA in classical murine dendritic cells in vivo.

ABSTRACT: Ciita was discovered for its role in regulating transcription of major histocompatibility complex class II (MHCII) genes. Subsequently, CIITA was predicted to control many other genes based on reporter and ChIP-seq analysis but few such predictions have been verified in vivo using Ciita^-/- mice. Testing these predictions for classical dendritic cells (cDCs) has been particularly difficult, since Ciita^-/- mice lack MHCII expression required to identify cDCs. However, recent identification of the cDC-specific transcription factor Zbtb46 allows the identification of cDCs independently of MHCII expression. We crossed Zbtb46^gfp mice onto the Ciita^-/- background and found that all cDC lineages developed in vivo in the absence of Ciita. We then compared

SUBMITTER: Anderson DA 

PROVIDER: S-EPMC5757839 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature