Reduced neuroprotective potential of the mesenchymal stromal cell secretome with ex vivo expansion, age and progressive multiple sclerosis.

ABSTRACT:

Background

Clinical trials using ex vivo expansion of autologous mesenchymal stromal cells (MSCs) are in progress for several neurological diseases including multiple sclerosis (MS). Given that environment alters MSC function, we examined whether in vitro expansion, increasing donor age and progressive MS affect the neuroprotective properties of the MSC secretome.

Methods

Comparative analyses of neuronal survival in the presence of MSC-conditioned medium (MSCcm) isolated from control subjects (C-MSCcm) and those with MS (MS-MSCcm) were performed following (1) trophic factor withdrawal and (2) nitric oxide-induced neurotoxicity.

Results

Reduced neuronal survival following trophic factor withdrawal was seen in association with increasing expansion of MSCs in vitro and M

SUBMITTER: Sarkar P 

PROVIDER: S-EPMC5758344 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature