A novel rat CVB1-VP1 monoclonal antibody 3A6 detects a broad range of enteroviruses.

ABSTRACT: Enteroviruses (EVs) are common RNA viruses that cause diseases ranging from rash to paralytic poliomyelitis. For example, EV-A and EV-C viruses cause hand-foot and mouth disease and EV-B viruses cause encephalitis and myocarditis, which can result in severe morbidity and mortality. While new vaccines and treatments for EVs are under development, methods for studying and diagnosing EV infections are still limited and therefore new diagnostic tools are required. Our aim was to produce and characterize new antibodies that work in multiple applications and detect EVs in tissues and in vitro. Rats were immunized with Coxsackievirus B1 capsid protein VP1 and hybridomas were produced. Hybridoma clones were selected based on their reactivity in different immunoassays. The most promising clone, 3A6

SUBMITTER: Saarinen NVV 

PROVIDER: S-EPMC5758616 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature