Surface functionalization of electrospun scaffolds using recombinant human decorin attracts circulating endothelial progenitor cells.

ABSTRACT: Decorin (DCN) is an important small leucine-rich proteoglycan present in the extracellular matrix (ECM) of many organs and tissues. Endothelial progenitor cells (EPCs) are able to interact with the surrounding ECM and bind to molecules such as DCN. Here, we recombinantly produced full-length human DCN under good laboratory practice (GLP) conditions, and after detailed immunological characterization, we investigated its potential to attract murine and human EPCs (mEPCs and hECFCs). Electrospun polymeric scaffolds were coated with DCN or stromal cell-derived factor-1 (SDF-1α) and were then dynamically cultured with both cell types. Cell viability was assessed via imaging flow cytometry. The number of captured cells was counted and compared with the non-coated controls. To characterize cell-s

SUBMITTER: Hinderer S 

PROVIDER: S-EPMC5758628 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature