A novel and quick PCR-based method to genotype mice with a leptin receptor mutation (db/db mice).

ABSTRACT: db/db mice is one of most widely used animal models in studying the cellular and molecular mechanisms of metabolic disorders, such as diabetes, hyperlipidemia, and obesity. The mice carry spontaneous point mutations in the gene encoding the leptin receptor, leading to leptin receptor inactivation. Since homozygous db/db mice are sterile, the maintenance of db/db mice requires breeding between heterozygous pairs, which makes genotyping essential for the identification of offspring. The aim of this study was to develop a quick and highly repeatable method for genotyping db/db mice, which comprised only three simple steps: genomic DNA is extracted from either tail tips or ear notches via alkaline lysis (∼20 min); samples are then subjected to tetra-primer amplification refractory mutation sys

SUBMITTER: Peng BY 

PROVIDER: S-EPMC5758667 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature