A specific form of prefibrillar aggregates that functions as a precursor of amyloid nucleation.

ABSTRACT: Non-fibrillar protein aggregates that appear in the earlier stages of amyloid fibril formation are sometimes considered to play a key role in amyloid nucleation; however, the structural features of these aggregates currently remain unclear. We herein identified a characteristic pathway of fibril formation by human insulin B chain, in which two major species of prefibrillar aggregates were identified. Based on the time-resolved tracking of this pathway with far-UV circular dichroism (CD) spectroscopy, dynamic light scattering (DLS), and ¹H-NMR spectroscopy, the first prefibrillar aggregate with a hydrodynamic diameter of approximately 70 nm accumulated concomitantly with the formation of a β-sheet structure, and the size further evolved to 130 nm with an additional structural dev

SUBMITTER: Yamamoto N 

PROVIDER: S-EPMC5758805 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature