Modulation of extracytoplasmic function (ECF) sigma factor promoter selectivity by spacer region sequence.

ABSTRACT: The ability of bacteria to adapt to stress depends on the conditional expression of specific sets of genes. Bacillus subtilis encodes seven extracytoplasmic function (ECF) sigma (σ) factors that regulate functions important for survival under conditions eliciting cell envelope stress. Of these, four have been studied in detail: σM, σW, σX and σV. These four σ factors recognize overlapping sets of promoters, although the sequences that determine this overlapping recognition are incompletely understood. A major role in promoter selectivity has been ascribed to the core -10 and -35 promoter elements. Here, we demonstrate that a homopolymeric T-tract motif, proximal to the -35 element, functions in combination with the core promoter sequences to determine selectivity for ECF sigma factors. Thi

SUBMITTER: Gaballa A 

PROVIDER: S-EPMC5758882 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature