Quantitative LC-MS/MS Glycomic Analysis of Biological Samples Using AminoxyTMT.

ABSTRACT: Protein glycosylation plays an important role in various biological processes, such as modification of protein function, regulation of protein-protein interactions, and control of turnover rates of proteins. Moreover, glycans have been considered as potential biomarkers for many mammalian diseases and development of aberrant glycosylation profiles is an important indicator of the pathology of a disease or cancer. Hence, quantitation is an important aspect of a comprehensive glycomics study. Although numerous MS-based quantitation strategies have been developed in the past several decades, some issues affecting sensitivity and accuracy of quantitation still exist, and the development of more effective quantitation strategies is still required. Aminoxy tandem mass tag (aminoxyTMT) reagents a

SUBMITTER: Zhou S 

PROVIDER: S-EPMC5759044 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature