The utility of DNA extracted from saliva for genome-wide molecular research platforms.

ABSTRACT:

Objective

The study aimed to investigate the suitability of DNA extracted from saliva for high throughput molecular genotyping and DNA methylation platforms by comparing its performance with that of DNA extracted from blood. The genome-wide methylation profile, using the Infinium HumanMethylation450 Beadchip array^® (Illumina, San Diego, CA), was measured for 20 DNA samples. Common genetic variation was measured, using the Infinium HumanCore Beadchip^® (Illumina, San Diego, CA) for 4 samples (matching samples from 2 people).

Results

DNA from blood and saliva returned genotyping call rates and reproducibility frequencies of > 99%. High-quality DNA methylation data was obtained from both saliva and blood DNA, with average detection p-values for each sample

SUBMITTER: Bruinsma FJ 

PROVIDER: S-EPMC5759806 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature