A new toolset for protein expression and subcellular localization studies in citrus and its application to citrus tristeza virus proteins.

ABSTRACT:

Background

Transient gene expression is a powerful tool to study gene function in plants. In citrus, Agrobacterium transformation is the method of choice for transient expression studies, but this method does not work efficiently with many gene constructs, and there is a need for a more robust transient expression system in citrus leaves. Biolistic particle delivery is an alternative to Agrobacterium transformation, and in some plants, such as Arabidopsis, gives higher transformation rates in leaf tissues than Agrobacterium.

Results

Here we describe an improved method for gene expression in epidermal cells of citrus leaves, using the Bio-Rad Helios gene-gun. Gene-gun bombardment of GFP-HDEL produced highly efficient gene expression in large number of cel

SUBMITTER: Levy A 

PROVIDER: S-EPMC5759842 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature