Synthesis and biophysical analysis of modified thymine-containing DNA oligonucleotides.

ABSTRACT: We report the synthesis of a 5-formyl-2'-deoxyuridine (5fU) phosphoramidite and the preparation of oligonucleotides comprising all known, naturally observed eukaryotic thymidine modifications. Biophysical characterization of the synthetic oligonucleotides indicates that 5fU, but not the other T-derivatives, can alter DNA structures.

SUBMITTER: Kawasaki F 

PROVIDER: S-EPMC5759927 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature