Chemical biology of genomic DNA: minimizing PCR bias.

ABSTRACT: The exquisite selectivity of chemical reactions enables the study of rare DNA bases. However, chemical modification of the genome can affect downstream analysis. We report a PCR bias caused by such modification, and exemplify a solution with the synthesis and characterization of a cleavable aldehyde-reactive biotinylation probe.

SUBMITTER: McInroy GR 

PROVIDER: S-EPMC5759930 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature