To what extent do structural changes in catalytic metal sites affect enzyme function?

ABSTRACT: About half of known enzymatic reactions involve metals. Enzymes belonging to the same superfamily often evolve to catalyze different reactions on the same structural scaffold. The work presented here investigates how functional differentiation, within superfamilies that contain metalloenzymes, relates to structural changes at the catalytic metal site. In general, when the catalytic metal site is unchanged across the enzymes of a superfamily, the functional differentiation within the superfamily tends to be low and the mechanism conserved. Conversely, all types of structural changes in the metal binding site are observed for superfamilies with high functional differentiation. Overall, the catalytic role of the metal ions appears to be one of the most conserved features of the enzyme mechani

SUBMITTER: Valasatava Y 

PROVIDER: S-EPMC5760197 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature