Functional properties of dopamine transporter oligomers after copper linking.

ABSTRACT: Although it is universally accepted that dopamine transporters (DATs) exist in monomers, dimers and tetramers (i.e. dimers of dimers), it is not known whether the oligomeric organization of DAT is a prerequisite for its ability to take up dopamine (DA), or whether each DAT protomer, the subunit of quaternary structure, functions independently in terms of DA translocation. In this study, copper phenanthroline (CuP) was used to selectively target surface DAT: increasing concentrations of CuP gradually cross-linked natural DAT dimers in LLC-PK₁ cells stably expressing hDAT and thereby reduced DA uptake functionality until all surface DATs were inactivated. DATs that were not cross-linked by CuP showed normal DA uptake with DA K_m at ~ 0.5 μM and DA efflux with basal and a

SUBMITTER: Zhen J 

PROVIDER: S-EPMC5760346 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature