Multiple mutations and overexpression of the MdaE7 carboxylesterase gene associated with male-linked malathion resistance in housefly, Musca domestica (Diptera: Muscidae).

ABSTRACT: Two unique housefly strains, MSS and N-MRS, were selected and used to clarify mechanisms of sex-associated malathion resistance in the housefly, Musca domestica. Compared with the lab-susceptible CSS strain, susceptible females and resistant males were observed in the malathion-susceptible MSS strain, while the malathion-resistant near-isogenic line, N-MRS, achieved similar resistance level between genders. Significant synergistic effect of the esterase-inhibitor DEF on resistant houseflies pointed to the important involvement of esterase in this specific malathion resistance. Examination of the carboxylesterase gene MdαE7 in malathion resistant housefly populations found seven, non-synonymous SNP mutations (Ser250-Thr, Trp251-Ser, Met303-Ile, Leu354-Phe, Ser357-Leu, Trp378-Arg and Ser383-

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC5760516 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature