HybTrack: A hybrid single particle tracking software using manual and automatic detection of dim signals.

ABSTRACT: Single particle tracking is a compelling technique for investigating the dynamics of nanoparticles and biological molecules in a broad range of research fields. In particular, recent advances in fluorescence microscopy have made single molecule tracking a prevalent method for studying biomolecules with a high spatial and temporal precision. Particle tracking algorithms have matured over the past three decades into more easily accessible platforms. However, there is an inherent difficulty in tracing particles that have a low signal-to-noise ratio and/or heterogeneous subpopulations. Here, we present a new MATLAB based tracking program which combines the benefits of manual and automatic tracking methods. The program prompts the user to manually locate a particle when an ambiguous situation o

SUBMITTER: Lee BH 

PROVIDER: S-EPMC5760724 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature