Molecular subtyping of European swine influenza viruses and scaling to high-throughput analysis.

ABSTRACT:

Background

Swine influenza is a respiratory infection of pigs that may have a significant economic impact in affected herds and pose a threat to the human population since swine influenza A viruses (swIAVs) are zoonotic pathogens. Due to the increasing genetic diversity of swIAVs and because novel reassortants or variants may become enzootic or have zoonotic implications, surveillance is strongly encouraged. Therefore, diagnostic tests and advanced technologies able to identify the circulating strains rapidly are critically important.

Results

Several reverse transcription real-time PCR assays (RT-qPCRs) were developed to subtype European swIAVs in clinical samples previously identified as containing IAV genome. The RT-qPCRs aimed to discriminate HA genes of four H1 genetic l

SUBMITTER: Bonin E 

PROVIDER: S-EPMC5761149 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature