Dietary fats promote functional and structural changes in the median eminence blood/spinal fluid interface-the protective role for BDNF.

ABSTRACT: BACKGROUND:The consumption of large amounts of dietary fats activates an inflammatory response in the hypothalamus, damaging key neurons involved in the regulation of caloric intake and energy expenditure. It is currently unknown why the mediobasal hypothalamus is the main target of diet-induced brain inflammation. We hypothesized that dietary fats can damage the median eminence blood/spinal fluid interface. METHODS:Swiss mice were fed on a high-fat diet, and molecular and structural studies were performed employing real-time PCR, immunoblot, immunofluorescence, transmission electron microscopy, and metabolic measurements. RESULTS:The consumption of a high fat diet was sufficient to increase the expression of inflammatory cytokines and brain-derived neurotrophic factor in the median eminen

SUBMITTER: Ramalho AF 

PROVIDER: S-EPMC5761204 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature