The kinetics of pre-mRNA splicing in the <i>Drosophila</i> genome and the influence of gene architecture.

ABSTRACT: Production of most eukaryotic mRNAs requires splicing of introns from pre-mRNA. The splicing reaction requires definition of splice sites, which are initially recognized in either intron-spanning ('intron definition') or exon-spanning ('exon definition') pairs. To understand how exon and intron length and splice site recognition mode impact splicing, we measured splicing rates genome-wide in Drosophila, using metabolic labeling/RNA sequencing and new mathematical models to estimate rates. We found that the modal intron length range of 60-70 nt represents a local maximum of splicing rates, but that much longer exon-defined introns are spliced even faster and more accurately. We observed unexpectedly low variation in splicing rates across introns in the same gene, suggesting the prese

SUBMITTER: Pai AA 

PROVIDER: S-EPMC5762160 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature