Identifying the dynamics of actin and tubulin polymerization in iPSCs and in iPSC-derived neurons.

ABSTRACT: The development of the nervous system requires cytoskeleton-mediated processes coordinating self-renewal, migration, and differentiation of neurons. It is not surprising that many neurodevelopmental problems and neurodegenerative disorders are caused by deficiencies in cytoskeleton-related genes. For this reason, we focus on the cytoskeletal dynamics in proliferating iPSCs and in iPSC-derived neurons to better characterize the underpinnings of cytoskeletal organization looking at actin and tubulin repolymerization studies using the cell permeable probes SiR-Actin and SiR-Tubulin. During neurogenesis, each neuron extends an axon in a complex and changing environment to reach its final target. The dynamic behavior of the growth cone and its capacity to respond to multiple spatial information

SUBMITTER: Magliocca V 

PROVIDER: S-EPMC5762308 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature