Enhanced biosynthesis of saponins by coronatine in cell suspension culture of Kalopanax septemlobus.

ABSTRACT: Kalopanax septemlobus is a medicinal woody species of the family Araliaceae, and the pharmaceutical properties of saponins obtained from K. septemlobus suggest that K. septemlobus has the potential to be a crude drug and dietary health supplement. In this study, we established cell suspension culture of K. septemlobus to develop a sustainable source of natura-ceuticals. Friable calli were used for establishing cell suspension culture. The maximum amount of total saponins (1.56 mg/60 ml suspension) was obtained during the 15th day of incubation, whereas the maximum capacity of saponin production was reached after day 6 (0.42 μg/mg of fresh weight). The total saponin production in the cell suspension of K. septemlobus was significantly increased by coronatine (COR) at 160% at a dose of 1 μM

SUBMITTER: Lee JK 

PROVIDER: S-EPMC5762595 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature