Assessment of three plastid DNA barcode markers for identification of Clinacanthus nutans (Acanthaceae).

ABSTRACT: This study was conducted to determine the feasibility of using three plastid DNA regions (matK, trnH-psbA, and rbcL) as DNA barcodes to identify the medicinal plant Clinacanthus nutans. In this study, C. nutans was collected at several different locations. Total genomic DNA was extracted, amplified by polymerase chain reaction (PCR), and sequenced using matK, trnH-psbA, and rbcL, primers. DNA sequences generated from PCR were submitted to the National Center for Biotechnology Information's (NCBI) GenBank. Identification of C. nutans was carried out using NCBI's Basic Local Alignment Search Tool (BLAST). The rbcL and trnH-psbA regions successfully identified C. nutans with sequencing rat

SUBMITTER: Ismail NZ 

PROVIDER: S-EPMC5762596 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature