Direct Molecular Fishing of New Protein Partners for Human Thromboxane Synthase.

ABSTRACT: Thromboxane synthase (TBXAS1) catalyzes the isomerization reaction of prostaglandin H2 producing thromboxane A2, the autocrine and paracrine factor in many cell types. A high activity and metastability by these arachidonic acid derivatives suggests the existence of supramolecular structures that are involved in the regulation of the biosynthesis and directed translocation of thromboxane to the receptor. The objective of this study was to identify TBXAS1 protein partners from human liver tissue lysate using a complex approach based on the direct molecular fishing technique, LC-MS/MS protein identification, and protein-protein interaction validation by surface plasmon resonance (SPR). As a result, 12 potential TBXAS1 protein partners were identified, including the components regulating cytos

SUBMITTER: Svirid AV 

PROVIDER: S-EPMC5762833 | biostudies-literature | 2017 Oct-Dec

REPOSITORIES: biostudies-literature