DNAJB9 Is a Specific Immunohistochemical Marker for Fibrillary Glomerulonephritis.

ABSTRACT:

Introduction

Fibrillary glomerulonephritis (FGN) is a rare disease with unknown pathogenesis and a poor prognosis. Until now, the diagnosis of this disease has required demonstration of glomerular deposition of randomly oriented fibrils by electron microscopy that are Congo red negative and stain with antisera to Igs. We recently discovered a novel proteomic tissue biomarker for FGN, namely, DNAJB9.

Methods

In this work, we developed DNAJB9 immunohistochemistry and tested its sensitivity and specificity for the diagnosis of FGN. This testing was performed on renal biopsy samples from patients with FGN (n = 84), amyloidosis (n = 21), a wide variety of non-FGN glomerular diseases (n = 98), and healthy subjects (n = 11). We also performed immunoelectron microscopy to determine

SUBMITTER: Nasr SH 

PROVIDER: S-EPMC5762944 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature