Heterogeneous Kinetics in the Functionalization of Single Plasmonic Nanoparticles.

ABSTRACT: The functionalization of gold nanoparticles with DNA has been studied extensively in solution; however, these ensemble measurements do not reveal particle-to-particle differences. Here we study the functionalization of gold nanorods with thiolated single-stranded DNA (ssDNA) at the single-particle level. We exploit the sensitivity of the plasmon resonance to the local refractive index to study the functionalization in real time using single-particle spectroscopy. We find particle-to-particle variations of the plasmon shift that are attributed to the particle size distribution and variations in ssDNA coverage. We find that the ssDNA coverage varies by ∼10% from particle to particle, beyond the expected variation due to Poisson statistics. Surprisingly, we find binding rates that differ from

SUBMITTER: Horacek M 

PROVIDER: S-EPMC5763282 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature