Measurement of ¹³ C turnover into glutamate and glutamine pools in brain tumor patients.

ABSTRACT: Malignant brain tumors are known to utilize acetate as an alternate carbon source in the citric acid cycle for their bioenergetics. ¹³ C NMR-based isotopomer analysis has been used to measure turnover of ¹³ C-acetate carbons into glutamate and glutamine pools in tumors. Plasma from the patients infused with [1,2-¹³ C]acetate further revealed the presence of ¹³ C isotopomers of glutamine, glucose, and lactate in the circulation that were generated due to metabolism of [1,2-¹³ C]acetate by peripheral organs. In the tumor cells, [4-¹³ C] and [3,4-¹³ C]glutamate and glutamine isotopomers were generated from blood-borne ¹³ C-labeled glucose and lactate which were formed due to [1,2-¹³ C[acetate me

SUBMITTER: Pichumani K 

PROVIDER: S-EPMC5763554 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature