Glycosyltransferase engineering for carbohydrate synthesis.

ABSTRACT: Glycosyltransferases (GTs) are powerful tools for the synthesis of complex and biologically-important carbohydrates. Wild-type GTs may not have all the properties and functions that are desired for large-scale production of carbohydrates that exist in nature and those with non-natural modifications. With the increasing availability of crystal structures of GTs, especially those in the presence of donor and acceptor analogues, crystal structure-guided rational design has been quite successful in obtaining mutants with desired functionalities. With current limited understanding of the structure-activity relationship of GTs, directed evolution continues to be a useful approach for generating additional mutants with functionality that can be screened for in a high-throughput format. Mutating t

SUBMITTER: McArthur JB 

PROVIDER: S-EPMC5764748 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature