Evaluation of the periodontal regenerative properties of patterned human periodontal ligament stem cell sheets.

ABSTRACT:

Purpose

The aim of this study was to determine the effects of patterned human periodontal ligament stem cell (hPDLSC) sheets fabricated using a thermoresponsive substratum.

Methods

In this study, we fabricated patterned hPDLSC sheets using nanotopographical cues to modulate the alignment of the cell sheet.

Results

The hPDLSCs showed rapid monolayer formation on various surface pattern widths. Compared to cell sheets grown on flat surfaces, there were no significant differences in cell attachment and growth on the nanopatterned substratum. However, the patterned hPDLSC sheets showed higher periodontal ligamentogenesis-related gene expression in early stages than the unpatterned cell sheets.

Conclusions

This experiment confirmed that patterned cell sheets provide

SUBMITTER: Kim JH 

PROVIDER: S-EPMC5764766 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature