Microwave measurement of giant unilamellar vesicles in aqueous solution.

ABSTRACT: A microwave technique is demonstrated to measure floating giant unilamellar vesicle (GUV) membranes in a 25 μm wide and 18.8 μm high microfluidic channel. The measurement is conducted at 2.7 and 7.9 GHz, at which a split-ring resonator (SRR) operates at odd modes. A 500 nm wide and 100 μm long SRR split gap is used to scan GUVs that are slightly larger than 25 μm in diameter. The smaller fluidic channel induces flattened GUV membrane sections, which make close contact with the SRR gap surface. The used GUVs are synthesized with POPC (16:0-18:1 PC 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine), SM (16:0 Egg Sphingomyelin) and cholesterol at different molecular compositions. It is shown that SM and POPC bilayers have different dielectric permittivity values, which also change with measure

SUBMITTER: Cui Y 

PROVIDER: S-EPMC5764977 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature