A set of synthetic versatile genetic control elements for the efficient expression of genes in Actinobacteria.

ABSTRACT: The design and engineering of secondary metabolite gene clusters that are characterized by complicated genetic organization, require the development of collections of well-characterized genetic control elements that can be reused reliably. Although a few intrinsic terminators and RBSs are used routinely, their translation and termination efficiencies have not been systematically studied in Actinobacteria. Here, we analyzed the influence of the regions surrounding RBSs on gene expression in these bacteria. We demonstrated that inappropriate RBSs can reduce the expression efficiency of a gene to zero. We developed a genetic device - an in vivo RBS-selector - that allows selection of an optimal RBS for any gene of interest, enabling rational control of the protein expression level. In additio

SUBMITTER: Horbal L 

PROVIDER: S-EPMC5765039 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature