Low amounts of dietary fibre increase in vitro production of short-chain fatty acids without changing human colonic microbiota structure.

ABSTRACT: This study investigated the effect of various prebiotics (indigestible dextrin, α-cyclodextrin, and dextran) on human colonic microbiota at a dosage corresponding to a daily intake of 6 g of prebiotics per person (0.2% of dietary intake). We used an in vitro human colonic microbiota model based on batch fermentation starting from a faecal inoculum. Bacterial 16S rRNA gene sequence analysis showed that addition of 0.2% prebiotics did not change the diversity and composition of colonic microbiota. This finding coincided with results from a clinical study showing that the microbiota composition of human faecal samples remained unchanged following administration of 6 g of prebiotics over seven days. However, compared to absence of prebiotics, their addition reduced the pH and increased the gen

SUBMITTER: Sasaki D 

PROVIDER: S-EPMC5765155 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature