Identification of a specific peptide binding to colon cancer cells from a phage-displayed peptide library.

ABSTRACT:

Background

New molecular probes are essential for early colon cancer diagnosis. A phage-display screening was performed to select novel binding peptides for early colon cancer imaging detection.

Methods

A human colon cancer cell line (COLO320HSR) and a normal human intestinal epithelial cell line (NCM460) were used for subtractive screening with a phage peptide library. The positive peptides were identified, and their binding capacities were confirmed by confocal immunofluorescence both in human colon cancer cells and in biopsy specimens. The sequences were further analysed for homology and the existing mimotopes by the BLAST algorithm and the MimoDB database.

Results

A peptide termed as CBP-DWS, which was demonstrated to be capable of binding to a panel of human colo

SUBMITTER: Hou L 

PROVIDER: S-EPMC5765222 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature