Targeting de novo lipogenesis as a novel approach in anti-cancer therapy.

ABSTRACT: BACKGROUND:Although altered membrane physiology has been discussed within the context of cancer, targeting membrane characteristics by drugs being an attractive therapeutic strategy has received little attention so far. METHODS:Various acetyl-CoA carboxylase 1 (ACC1), and fatty acid synthase (FASN) inhibitors (like Soraphen A and Cerulenin) as well as genetic knockdown approaches were employed to study the effects of disturbed phospholipid composition on membrane properties and its functional impact on cancer progression. By using state-of-the-art methodologies such as LC-MS/MS, optical tweezers measurements of giant plasma membrane vesicles and fluorescence recovery after photobleaching analysis, membrane characteristics were examined. Confocal laser scanning microscopy, proximity ligatio

SUBMITTER: Stoiber K 

PROVIDER: S-EPMC5765225 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature