Mechanisms of inactivation of the tumour suppressor gene RHOA in colorectal cancer.

ABSTRACT:

Background

Reduced RHOA signalling has been shown to increase the growth/metastatic potential of colorectal tumours. However, the mechanisms of inactivation of RHOA signalling in colon cancer have not been characterised.

Methods

A panel of colorectal cancer cell lines and large cohorts of primary tumours were used to investigate the expression and activity of RHOA, as well as the presence of RHOA mutations/deletions and promoter methylation affecting RHOA. Changes in RHOA expression were assessed by western blotting and qPCR after modulation of microRNAs, SMAD4 and c-MYC.

Results

We show here that RHOA point mutations and promoter hypermethylation do not significantly contribute to the large variability of RHOA expression observed among colorectal tumours. However, RH

SUBMITTER: Dopeso H 

PROVIDER: S-EPMC5765235 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature