Genotyping by Sequencing in Almond: SNP Discovery, Linkage Mapping, and Marker Design.

ABSTRACT: In crop plant genetics, linkage maps provide the basis for the mapping of loci that affect important traits and for the selection of markers to be applied in crop improvement. In outcrossing species such as almond (Prunus dulcis Mill. D. A. Webb), application of a double pseudotestcross mapping approach to the F₁ progeny of a biparental cross leads to the construction of a linkage map for each parent. Here, we report on the application of genotyping by sequencing to discover and map single nucleotide polymorphisms in the almond cultivars "Nonpareil" and "Lauranne." Allele-specific marker assays were developed for 309 tag pairs. Application of these assays to 231 Nonpareil × Lauranne F₁ progeny provided robust linkage maps for each parent. Analysis of phenotypic

SUBMITTER: Goonetilleke SN 

PROVIDER: S-EPMC5765344 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature