RNA-Sequencing of Drosophila melanogaster Head Tissue on High-Sugar and High-Fat Diets.

ABSTRACT: Obesity has been shown to increase risk for cardiovascular disease and type-2 diabetes. In addition, it has been implicated in aggravation of neurological conditions such as Alzheimer's. In the model organism Drosophila melanogaster, a physiological state mimicking diet-induced obesity can be induced by subjecting fruit flies to a solid medium disproportionately higher in sugar than protein, or that has been supplemented with a rich source of saturated fat. These flies can exhibit increased circulating glucose levels, increased triglyceride content, insulin-like peptide resistance, and behavior indicative of neurological decline. We subjected flies to variants of the high-sugar diet, high-fat diet, or normal (control) diet, followed by a total RNA extraction from fly heads of each d

SUBMITTER: Hemphill W 

PROVIDER: S-EPMC5765356 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature