Quantitative proteomics of a B₁₂ -dependent alga grown in coculture with bacteria reveals metabolic tradeoffs required for mutualism.

ABSTRACT: The unicellular green alga Lobomonas rostrata requires an external supply of vitamin B₁₂ (cobalamin) for growth, which it can obtain in stable laboratory cultures from the soil bacterium Mesorhizobium loti in exchange for photosynthate. We investigated changes in protein expression in the alga that allow it to engage in this mutualism. We used quantitative isobaric tagging (iTRAQ) proteomics to determine the L. rostrata proteome grown axenically with B₁₂ supplementation or in coculture with M. loti. Data are available via ProteomeXchange (PXD005046). Using the related Chlamydomonas reinhardtii as a reference genome, 588 algal proteins could be identified. Enzymes of amino acid biosynthesis were higher in coculture than in axenic culture, and this was reflected in incr

SUBMITTER: Helliwell KE 

PROVIDER: S-EPMC5765456 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature