Exploring cellular behavior under transient gene expression and its impact on mAb productivity and Fc-glycosylation.

ABSTRACT: Transient gene expression (TGE) is a methodology employed in bioprocessing for the fast provision of recombinant protein material. Mild hypothermia is often introduced to overcome the low yield typically achieved with TGE and improve specific protein productivity. It is therefore of interest to examine the impact of mild hypothermic temperatures on both the yield and quality of transiently expressed proteins and the relationship to changes in cellular processes and metabolism. In this study, we focus on the ability of a Chinese hamster ovary cell line to galactosylate a recombinant monoclonal antibody (mAb) product. Through experimentation and flux balance analysis, our results show that TGE in mild hypothermic conditions led to a 76% increase in q_P compared to TGE at 36.5°C in

SUBMITTER: Sou SN 

PROVIDER: S-EPMC5765507 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature