Improving amphibian genomic resources: a multitissue reference transcriptome of an iconic invader.

ABSTRACT:

Background

Cane toads (Rhinella marina) are an iconic invasive species introduced to 4 continents and well utilized for studies of rapid evolution in introduced environments. Despite the long introduction history of this species, its profound ecological impacts, and its utility for demonstrating evolutionary principles, genetic information is sparse. Here we produce a de novo transcriptome spanning multiple tissues and life stages to enable investigation of the genetic basis of previously identified rapid phenotypic change over the introduced range.

Findings

Using approximately 1.9 billion reads from developing tadpoles and 6 adult tissue-specific cDNA libraries, as well as a transcriptome assembly pipeline encompassing 100 separate de novo assemblies, we constructed 62 202

SUBMITTER: Richardson MF 

PROVIDER: S-EPMC5765561 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature