De novo assembly of the complex genome of Nippostrongylus brasiliensis using MinION long reads.

ABSTRACT:

Background

Eukaryotic genome assembly remains a challenge in part due to the prevalence of complex DNA repeats. This is a particularly acute problem for holocentric nematodes because of the large number of satellite DNA sequences found throughout their genomes. These have been recalcitrant to most genome sequencing methods. At the same time, many nematodes are parasites and some represent a serious threat to human health. There is a pressing need for better molecular characterization of animal and plant parasitic nematodes. The advent of long-read DNA sequencing methods offers the promise of resolving complex genomes.

Results

Using Nippostrongylus brasiliensis as a test case, applying improved base-calling algorithms and assembly methods, we demonstrate the feasibility of de

SUBMITTER: Eccles D 

PROVIDER: S-EPMC5765664 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature